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Characterization and application of microwave plasma on wound healing
Smejkalová, Kateřina ; Skoumalová, Petra (referee) ; Krčma, František (advisor)
The aim of the Master thesis is the investigation of the influence of microwave discharge for skin wound healing. Microwave discharge used for this work was argon microwave plasma generated by the surface wave and direct vortex torch. The theoretical part is focused on basic information about plasma and processes that occur in plasma discharge under specific conditions. Plasma generates various active particles such as hydroxyl radicals, nitric oxide radicals, excited nitrogen molecules, atomic nitrogen, argon and oxygen. All of these particles together with plasma generated photons are usable in biomedical applications and summary of them is shown in the theoretical part. The experimental part is focused on the comparison of torch discharge and microwave plasma generated surface wave in skin wound healing. The model wounds on laboratory mousses were treated by plasma and wound healing was examined during 3 weeks after the plasma treatment. Both plasma systems showed healing acceleration. Application of torch discharge was proved to be the most effective method in the healing of skin defects. Additionally, determination of active particles was taken by optical emission spectroscopy. Based on these measurements, plasma parameters were determined: electron temparutare, rotational and vibrational temperatures. To determine role of different plasma active species, the treatment of indigo coloured artificial skin model was treated under various conditions by both plasma systems. Results show that the direct interaction between plasma particles is the main effect, role of radiation, only, is more or less negligible. Finally, the plasma vortex system was visualized using fast camera at selected powers and gas flows.
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Characterization of microwave plasma jet generated in argon-oxygen mixtures
Smejkalová, Kateřina ; Mazánková, Věra (referee) ; Krčma, František (advisor)
The optical diagnostics of microwave plasma torch operating at the frequency of 2,45 GHz is a subject of this bachelor thesis. Microwave plasma is generated using the surfatron resonator in argon with oxygen admixture (up to 1,7 %) at the constant argon flow of 3 Slm. The theoretical part gives the basic information about plasma and processes running under plasma conditions. Various active particles such as hydroxyl radicals, nitric oxides, excited nitrogen molecules, nitrogen, argon and oxygen atoms are generated in the discharge. All these particles together with the plasma generated photons can be used in biomedical applications that are briefly reviewed in the theoretical part, too. The visual observation of oxygen admixture on the discharge and the optical emission spectroscopy based determination of the active particles presence along the discharge and post-discharge axis is the subject of experimental part. Based on it, the selected plasma parameters like electron, rotational and vibrational temperatures were calculated.
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Characterization and application of microwave plasma on wound healing
Smejkalová, Kateřina ; Skoumalová, Petra (referee) ; Krčma, František (advisor)
The aim of the Master thesis is the investigation of the influence of microwave discharge for skin wound healing. Microwave discharge used for this work was argon microwave plasma generated by the surface wave and direct vortex torch. The theoretical part is focused on basic information about plasma and processes that occur in plasma discharge under specific conditions. Plasma generates various active particles such as hydroxyl radicals, nitric oxide radicals, excited nitrogen molecules, atomic nitrogen, argon and oxygen. All of these particles together with plasma generated photons are usable in biomedical applications and summary of them is shown in the theoretical part. The experimental part is focused on the comparison of torch discharge and microwave plasma generated surface wave in skin wound healing. The model wounds on laboratory mousses were treated by plasma and wound healing was examined during 3 weeks after the plasma treatment. Both plasma systems showed healing acceleration. Application of torch discharge was proved to be the most effective method in the healing of skin defects. Additionally, determination of active particles was taken by optical emission spectroscopy. Based on these measurements, plasma parameters were determined: electron temparutare, rotational and vibrational temperatures. To determine role of different plasma active species, the treatment of indigo coloured artificial skin model was treated under various conditions by both plasma systems. Results show that the direct interaction between plasma particles is the main effect, role of radiation, only, is more or less negligible. Finally, the plasma vortex system was visualized using fast camera at selected powers and gas flows.
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Vliv sucha a ošetření semen studeným plazmatem na stavbu a funkci fotosyntetického aparátu brukve řepky olejky
HANZAL, Jaroslav
In the period of global climate changes, the resistance of significant agricultural crops to drought has become from an economical point of view a very important topic. In order to achieve maximum productivity of agricultural crops, it is necessary to search for new technological approaches to enhance this resistance. Currently, one of the actual topics in this area is the possibility of seed treatment with cold plasma. The aim of this work is to reveal the degree of influence of cold-plasma treatment of seeds on the stress response of plants that had been grown under the influence of drought. The oilseed rape was chosen for this experiment intentionally because it remains a substantial agricultural crop with a huge variety of usage. The plants of rapeseed had been grown in fully-controlled conditions in a growth chamber. The selected photosynthetic characteristics had been checked in regular intervals and the stress response was evaluated on two variables: plants grown from seeds treated with cold plasma and plants grown from commonly used seeds. In total, three measurements of chlorophyll fluorescence and photosynthetic pigment content have been conducted. The first measurement in the beginning of development of the seedlings showed better drought resistance in nontreated seedlings but in the following second and third measurements it was vice versa: cold-plasma treated plants survived better in dry conditions. Results of the analysis of photosynthetic pigments suggest that the cold plasma treatment can obviously increase the resistance of seedlings of rape especially in conditions of long-term drought.
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Characterization of microwave plasma jet generated in argon-oxygen mixtures
Smejkalová, Kateřina ; Mazánková, Věra (referee) ; Krčma, František (advisor)
The optical diagnostics of microwave plasma torch operating at the frequency of 2,45 GHz is a subject of this bachelor thesis. Microwave plasma is generated using the surfatron resonator in argon with oxygen admixture (up to 1,7 %) at the constant argon flow of 3 Slm. The theoretical part gives the basic information about plasma and processes running under plasma conditions. Various active particles such as hydroxyl radicals, nitric oxides, excited nitrogen molecules, nitrogen, argon and oxygen atoms are generated in the discharge. All these particles together with the plasma generated photons can be used in biomedical applications that are briefly reviewed in the theoretical part, too. The visual observation of oxygen admixture on the discharge and the optical emission spectroscopy based determination of the active particles presence along the discharge and post-discharge axis is the subject of experimental part. Based on it, the selected plasma parameters like electron, rotational and vibrational temperatures were calculated.
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